HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
نویسندگان
چکیده
منابع مشابه
HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP.
In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a smal...
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Mosayebi M1, Dalimi Asl A2, Moazeni M3, Mosayebi Gh4 1. Ph.D Student, Department of Parasitology, Faculty of medicine, Tarbiat Modarres University 2. Professor, Department of Parasitology, Faculty of medicine, Tarbiat Modarres University 3. Professor, Department of Immunology, Faculty of medicine, Tarbiat Modarres University 4. Assistant professor, Department of Immunology, Faculty of medici...
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2001
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2001000700005